Bienvenu J G, Tanguay J F, Chauvet P, Merhi Y
Laboratory of Experimental Pathology, Montreal Heart Institute, University of Montreal, Q.C., Canada.
J Vasc Res. 2001 Mar-Apr;38(2):153-62. doi: 10.1159/000051042.
Platelet and neutrophil interactions with injured vascular wall may contribute to restenosis. Their importance was mainly examined following balloon injury of intact arteries. However, dilation of diseased arteries is clinically more relevant and may elicit different responses. We investigated the relationship between platelets and neutrophil adhesion, neointima formation and P-selectin expression on damaged arteries after repeated balloon injury. In an acute single-injury model, 8 pigs were subjected to bilateral carotid angioplasty and sacrificed 1 h later. In a chronic model, 19 pigs were subjected to similar procedures and allowed to recover for 4 weeks; then 18 arteries were redilated at the same previously injured sites (double injury) while the remaining arteries were not redilated and used to investigate the extent and the adhesive properties of the neointima. After single injury, (51)Cr-platelet adhesion (x10(6)/cm(2)) increased significantly from 3.8 +/- 0.6 to 45.9 +/- 6.5 (p < 0.05) on mildly and deeply injured segments, respectively, and were statistically similar after double injury. After single injury, (111)In-neutrophil adhesion (x10(3)/cm(2)) increased from 226.6 +/- 45.5 to 512.5 +/- 70.3 (p < 0.05) on mildly and deeply injured segments, and were significantly higher (p < 0.05) after double injury (mild: 1,289.1 +/- 227.9 and deep: 2,411.8 +/- 333.9). As well, the neo-endothelium expresses P-selectin at 4 weeks and platelet and neutrophil adhesion was directly related to neointimal growth. These results, which indicate ongoing proinflammatory processes 1 month post-angioplasty, suggest that neutrophils may participate in the progression of restenosis.
血小板和中性粒细胞与受损血管壁的相互作用可能导致再狭窄。它们的重要性主要在完整动脉球囊损伤后进行研究。然而，病变动脉的扩张在临床上更具相关性，并且可能引发不同的反应。我们研究了反复球囊损伤后受损动脉上血小板与中性粒细胞黏附、新生内膜形成和P-选择素表达之间的关系。在急性单次损伤模型中，8头猪接受双侧颈动脉血管成形术，并在1小时后处死。在慢性模型中，19头猪接受类似手术并恢复4周；然后在相同的先前损伤部位对18条动脉进行再次扩张（双重损伤），而其余动脉不进行再次扩张，用于研究新生内膜的范围和黏附特性。单次损伤后，轻度和深度损伤节段上的（51）铬标记血小板黏附（×10（6）/平方厘米）分别从3.8±0.6显著增加至45.9±6.5（p<0.05），双重损伤后在统计学上相似。单次损伤后，轻度和深度损伤节段上的（111）铟标记中性粒细胞黏附（×10（3）/平方厘米）从226.6±45.5增加至512.5±70.3（p<0.05），双重损伤后显著更高（p<0.05）（轻度：1289.1±227.9，深度：2411.8±333.9）。同样，新生内膜在4周时表达P-选择素，血小板和中性粒细胞黏附与新生内膜生长直接相关。这些结果表明血管成形术后1个月存在持续的促炎过程，提示中性粒细胞可能参与再狭窄的进展。
