Dresser M E, Hamilton A E
Cytogenet Cell Genet. 1979;24(3):160-7. doi: 10.1159/000131372.
In this report we compare the karyotype of Lemus fulvus fulvus (2n=60) with that of Cheirogaleus medius (2n=66), a species thought to retain the ancestral lemur karyotype. A culture technique was designed specifically for lemur lymphocytes to facilitate description of the complete karyotypes using G--banding, C-banding, and Ag-AS staining for nucleolus organizer regions (NOR's). Different G-banding patterns in three chromosome pairs and different NOR-bearing chromosomes between the two species, as well as additional chromosomes and interstitial C-bands in C. medius, suggest that the chromosome complement of C. medius may not perfectly reflect the ancestral morphology. However, allowing for a Robertsonian centric fusion and a pericentric inversion, the G-banding patterns of 27 of the 32 autosomal pairs of C. medius are indistinguishable from those of L. fulvus fulvus. This constitutes strong justification for assigning these chromosomes to the ancestral lemur karyotype.
在本报告中，我们将褐美狐猴（2n = 60）的核型与中叉狐猴（2n = 66）的核型进行了比较，中叉狐猴被认为保留了狐猴的原始核型。我们专门设计了一种针对狐猴淋巴细胞的培养技术，以便利用G带、C带以及用于核仁组织区（NOR）的银染 - 反染技术来描述完整的核型。两个物种的三对染色体中不同的G带模式、不同的携带NOR的染色体，以及中叉狐猴中额外的染色体和中间C带，表明中叉狐猴的染色体组可能无法完美反映原始形态。然而，考虑到罗伯逊着丝粒融合和臂间倒位，中叉狐猴32对常染色体中的27对的G带模式与褐美狐猴的无法区分。这为将这些染色体归为原始狐猴核型提供了有力依据。
