Dahm K, Nielsen P J, Müller A M
Max-Planck Institut für Immunobiologie, 79108 Freiburg, Germany.
Genomics. 2001 Apr 15;73(2):194-202. doi: 10.1006/geno.2000.6480.
The origin and expansion of hematopoietic progenitor and stem cells during fetal development and their differentiation into mature effector cells are thought to be driven by the activation of developmental stage- and cell-lineage-specific genes. To gain further insight into the molecular mechanisms regulating the expansion and differentiation of fetal hematopoietic progenitor and stem cells, we performed differential display RT-PCR analysis on fractionated murine E12 fetal liver cells. We identified a novel transcript predicted to encode a protein of 305 amino acids with a calculated molecular mass of 35 kDa, containing a charged domain and three putative C(3)H-type zinc fingers. The fetal liver zinc-finger protein 1 (Fliz1) transcript is approximately 1.8 kb and is variably expressed both during embryogenesis and in adult tissues. Fliz1 expression was detected in discrete cell foci in the fetal liver and in LIN(-)/ckit(+) cells. Nuclear localization studies revealed that Fliz1 is targeted to the nucleus. Thus, Fliz1 is a newly identified nuclear protein expressed in hematopoietic progenitor cells of the developing fetal liver.
造血祖细胞和干细胞在胎儿发育过程中的起源、扩增以及它们分化为成熟效应细胞的过程，被认为是由发育阶段和细胞谱系特异性基因的激活所驱动的。为了进一步深入了解调节胎儿造血祖细胞和干细胞扩增与分化的分子机制，我们对分离的小鼠E12期胎儿肝细胞进行了差异显示逆转录聚合酶链反应（RT-PCR）分析。我们鉴定出一种新的转录本，预计编码一个由305个氨基酸组成、计算分子量为35 kDa的蛋白质，该蛋白质含有一个带电荷结构域和三个假定的C(3)H型锌指。胎儿肝脏锌指蛋白1（Fliz1）转录本约为1.8 kb，在胚胎发育过程中和成年组织中均有不同程度的表达。在胎儿肝脏的离散细胞灶以及LIN(-)/ckit(+)细胞中检测到了Fliz1的表达。核定位研究表明Fliz1定位于细胞核。因此，Fliz1是一种新鉴定出的在发育中的胎儿肝脏造血祖细胞中表达的核蛋白。
