Xiao S Y, Zhang H, Guzman H, Tesh R B
Department of Pathology and Center for Tropical Diseases, University of Texas Medical Branch, Galveston, TX 77555-0588, USA.
J Infect Dis. 2001 May 15;183(10):1437-44. doi: 10.1086/320200. Epub 2001 Apr 17.
Subadult and adult hamsters were inoculated intraperitoneally with 10(6) TCID(50) of yellow fever (YF) virus (Jimenez strain). Four animals from each group were subjected daily to histologic examination for 9 days. The liver showed spotty necrosis on day 3 after infection, which was followed by steatosis and focally confluent necrosis. In surviving hamsters, hepatocyte regeneration began on day 8, which was accompanied by decreasing steatosis. The spleen initially exhibited lymphoid hyperplasia, which was followed by lymphoid depletion and increased phagocytosis by splenic macrophages. Focal pancreatic acinar necrosis and spotty adrenal cortical necrosis were seen transiently between days 5 and 7. Viral antigen was detected immunohistochemically in the liver and the spleen. TUNEL analysis showed a dynamic change of hepatocyte necrapoptosis, with activity corresponding to the severity of disease. The histopathologic changes were more severe in younger (subadult) animals. The YF-hamster model appears to be an accurate and inexpensive experimental system for studying the pathophysiology and treatment of YF.
将亚成体和成年仓鼠经腹腔接种10(6) 半数组织培养感染剂量（TCID(50)）的黄热病毒（YF）（希门尼斯毒株）。每组4只动物，连续9天每天进行组织学检查。感染后第3天肝脏出现点状坏死，随后出现脂肪变性和局灶性融合性坏死。在存活的仓鼠中，肝细胞再生于第8天开始，同时脂肪变性减轻。脾脏最初表现为淋巴组织增生，随后出现淋巴细胞耗竭，脾巨噬细胞吞噬作用增强。在第5天至第7天期间短暂出现局灶性胰腺腺泡坏死和点状肾上腺皮质坏死。通过免疫组织化学方法在肝脏和脾脏中检测到病毒抗原。TUNEL分析显示肝细胞坏死凋亡呈动态变化，其活性与疾病严重程度相关。组织病理学变化在较年轻（亚成体）动物中更为严重。黄热仓鼠模型似乎是研究黄热病理生理学和治疗方法的一种准确且廉价的实验系统。
