Robinson D A, Edwards K M, Waites K B, Briles D E, Crain M J, Hollingshead S K
Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
J Infect Dis. 2001 May 15;183(10):1501-7. doi: 10.1086/320194. Epub 2001 Apr 17.
To determine whether nasopharyngeal carriage isolates of Streptococcus pneumoniae are of the same genetic background as isolates that caused invasive disease in one community, IS1167 and boxA genotypes were obtained for 182 pneumococcal isolates from children living in central Tennessee. The isolates represented 70 combined IS1167-boxA genotypes. The genotypic diversity of the invasive isolates was significantly less than that of the total population (P=.003). Most of the carriage isolates belonged to genotypes unique to carriage, whereas most of the invasive isolates belonged to genotypes common to carriage and disease (P=.02). Monte Carlo simulations showed a greater number of genotypes unique to carriage than can be explained by chance (P<.05 in all cases). Two genotypes were identified by multilocus sequence typing as members of globally disseminated clones, and one such genotype that was strictly carriage in this sample caused disease in other studies. Thus, clones can have different propensities for carriage and invasion.
为确定肺炎链球菌的鼻咽部携带分离株是否与在某一社区引起侵袭性疾病的分离株具有相同的遗传背景，我们获取了田纳西州中部地区182名儿童肺炎球菌分离株的IS1167和boxA基因型。这些分离株代表了70种组合的IS1167 - boxA基因型。侵袭性分离株的基因型多样性显著低于总体人群（P = 0.003）。大多数携带分离株属于携带特有的基因型，而大多数侵袭性分离株属于携带和疾病共有的基因型（P = 0.02）。蒙特卡罗模拟显示，携带特有的基因型数量比偶然因素所能解释的要多（所有情况P < 0.05）。通过多位点序列分型鉴定出两种基因型为全球传播克隆的成员，在本样本中严格为携带型的一种此类基因型在其他研究中导致了疾病。因此，克隆对于携带和侵袭可能具有不同的倾向。
