Distler A M, Allison J
Department of Chemistry, Michigan State University, East Lansing 48824-1322, USA.
J Am Soc Mass Spectrom. 2001 Apr;12(4):456-62. doi: 10.1016/S1044-0305(01)00212-4.
5-Methoxysalicylic acid (MSA) is demonstrated to be a useful matrix for matrix-assisted laser desorption/ionization time-of-flight (TOF) mass spectrometry of oligonucleotides, when desorption/ionization without fragmentation is desired. When MSA is combined with the additive spermine, the need for desalting is reduced. The MSA/spermine matrix yields linear TOF mass spectra with improved resolution, less fragmentation, and less intense alkali ion adduct peaks than those spectra obtained using 3-hydroxypicolinic acid and 6-aza-2-thiothymine with spermine or diammonium hydrogen citrate as additives. Instrumental conditions are discussed to improve the spectral resolution, specifically the use of longer delay times in the delayed-extraction ion source.
5-甲氧基水杨酸（MSA）被证明是寡核苷酸基质辅助激光解吸/电离飞行时间（TOF）质谱分析的一种有用基质，前提是需要无碎片化的解吸/电离。当MSA与添加剂精胺结合时，脱盐的需求会降低。与使用3-羟基吡啶甲酸和6-氮杂-2-硫代胸腺嘧啶并添加精胺或柠檬酸氢二铵作为添加剂所获得的质谱相比，MSA/精胺基质产生的线性TOF质谱具有更高的分辨率、更少的碎片化以及更弱的碱金属离子加合物峰。文中讨论了改善光谱分辨率的仪器条件，特别是在延迟提取离子源中使用更长的延迟时间。
