人胎儿睾丸中的α-抑制素和转铁蛋白。
Alfa-inhibin and transferrin in human fetal testis.
作者信息
Balboni G C, Barni T, Gloria L, Forti G, Vannelli G B
机构信息
Department of Human Anatomy and Histology, University of Florence, Italy.
出版信息
Ital J Anat Embryol. 1995;100 Suppl 1:519-24.
Aim of this research was to investigate the immunohistochemical localization of alfa-inhibin and transferrin in the human fetal testis. In addition, an attempt was made to identify the Sertoli cells in primary cell cultures of fetal testes by the demonstration of the mRNA for transferrin and the presence of the FSH receptor. 15 fetal testes (8-12 weeks) were utilized. The results of this study show: 1. In sections, immunoreactivity for alfa-inhibin was present in the interstitial cells, whereas transferrin was localized in Sertoli cells; 2. After 20 days of primary culture: a) the percentage of immunoreactive cells for both the substances was about 15%; b) the presence of FSH receptor and the expression of transferrin mRNA were observed in 12-15% of cells. These findings suggest: 1. alfa-inhibin and transferrin are present in the human fetal testis; 2. the expression of transferrin mRNA and the presence of the FSH receptor permit the identification of the Sertoli cells in primary cultures of fetal testes (8-12 weeks).
本研究的目的是调查α-抑制素和转铁蛋白在人胎儿睾丸中的免疫组织化学定位。此外，试图通过转铁蛋白mRNA的显示和FSH受体的存在来鉴定胎儿睾丸原代细胞培养中的支持细胞。使用了15个胎儿睾丸（8 - 12周）。本研究结果表明：1. 在切片中，α-抑制素的免疫反应性存在于间质细胞中，而转铁蛋白定位于支持细胞中；2. 原代培养20天后：a）两种物质的免疫反应性细胞百分比约为15%；b）在12 - 15%的细胞中观察到FSH受体的存在和转铁蛋白mRNA的表达。这些发现表明：1. α-抑制素和转铁蛋白存在于人胎儿睾丸中；2. 转铁蛋白mRNA的表达和FSH受体的存在允许在胎儿睾丸（8 - 12周）的原代培养中鉴定支持细胞。
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