东亚钳蝎毒素BmK M1的电生理学特性研究，BmK M1是一种来自东亚钳蝎毒液的α-样毒素。

Electrophysiological characterization of BmK M1, an alpha-like toxin from Buthus martensi Karsch venom.

作者信息

Goudet C, Huys I, Clynen E, Schoofs L, Wang D C, Waelkens E, Tytgat J

机构信息

Laboratory of Toxicology, University of Leuven, E. Van Evenstraat 4, B-3000 Leuven, Belgium.

出版信息

FEBS Lett. 2001 Apr 20;495(1-2):61-5. doi: 10.1016/s0014-5793(01)02365-1.

DOI:10.1016/s0014-5793(01)02365-1

PMID:11322948

Abstract

The present study investigates the electrophysiological actions of BmK M1, an alpha-like toxin purified from the venom of the scorpion Buthus martensi Karsch, on voltage-gated Na+ channels. Using the voltage clamp technique, we assessed the BmK M1 activity on the cardiac Na+ channel (hH1) functionally expressed in Xenopus oocytes. The main actions of the toxin are a concentration-dependent slowing of the inactivation process and a hyperpolarizing shift of the steady-state inactivation. This work is the first electrophysiological characterization of BmK M1 on a cloned Na+ channel, demonstrating that this toxin belongs to the class of scorpion alpha-toxins. Our results also show that BmK M1 can be considered as a cardiotoxin.

摘要

本研究调查了从东亚钳蝎毒液中纯化得到的α-样毒素BmK M1对电压门控性Na⁺通道的电生理作用。运用电压钳技术，我们评估了BmK M1对非洲爪蟾卵母细胞中功能性表达的心脏Na⁺通道（hH1）的活性。该毒素的主要作用是使失活过程呈浓度依赖性减慢以及稳态失活发生超极化偏移。这项工作是对BmK M1在克隆的Na⁺通道上的首次电生理特性描述，证明该毒素属于蝎α-毒素类别。我们的结果还表明BmK M1可被视为一种心脏毒素。