Nan H M, Kim H, Lim H S, Choi J K, Kawamoto T, Kang J W, Lee C H, Kim Y D, Kwon E H
Department of Preventive Medicine, College of Medicine, Chungbuk National University, South Korea.
Carcinogenesis. 2001 May;22(5):787-93. doi: 10.1093/carcin/22.5.787.
This study was undertaken to determine the effects of occupation, lifestyle and the genetic polymorphisms of cytochrome P450 1A1 (CYP1A1), cytochrome P450 2E1 (CYP2E1) and glutathione S-transferases micro1 (GSTM1) and 1 (GSTT1) on the concentrations of urinary 1-hydroxypyrene (1-OHP) and 2-naphthol among Korean coke oven workers and university students. The study subjects included 90 coke oven workers and 128 university students. A questionnaire was used to obtain detailed data about the work area, smoking habits and food intake of subjects. Associations between urinary polycyclic aromatic hydrocarbon (PAH) concentrations and occupation, smoking status, total airborne PAH level and genetic polymorphisms were tested. Urinary 1-OHP and 2-naphthol concentrations were higher in coke oven workers than in students and correlated significantly with work area. Urinary 2-naphthol concentrations increased with an increase in the level of cigarette smoking in students. Total airborne PAH level correlated with urinary 1-OHP concentration in coke oven workers. Urinary 1-OHP and 2-naphthol concentrations were higher in coke oven workers with the c1/c2 or c2/c2 genotype of CYP2E1 than in those with the c1/c1 genotype. Urinary 2-naphthol concentrations were higher in GSTM1-null workers than in GSTM1-positive workers. In multiple regression analysis CYP2E1 was a significant factor determining urinary 1-OHP concentrations in coke oven workers. CYP2E1 and GSTM1 were significant determinants for urinary 2-naphthol concentrations in coke oven workers and GSTM1 and smoking were prognosticators among university students. Urinary 1-OHP is a better indicator of occupational exposure to PAH in coke oven workers than 2-naphthol, whereas urinary 2-naphthol may be more sensitive for non-occupational inhalation exposure to PAH. In occupationally exposed populations CYP2E1 and GSTM1 appear to play an important role in the metabolism of pyrene and naphthalene. In individuals not occupationally exposed to PAHs GSTM1 and smoking seem to influence the urinary concentration of 2-naphthol.
本研究旨在确定职业、生活方式以及细胞色素P450 1A1（CYP1A1）、细胞色素P450 2E1（CYP2E1）、谷胱甘肽S-转移酶μ1（GSTM1）和θ1（GSTT1）的基因多态性对韩国炼焦炉工人和大学生尿中1-羟基芘（1-OHP）和2-萘酚浓度的影响。研究对象包括90名炼焦炉工人和128名大学生。通过问卷调查获取有关研究对象工作区域、吸烟习惯和食物摄入的详细数据。检测尿中多环芳烃（PAH）浓度与职业、吸烟状况、空气中总PAH水平及基因多态性之间的关联。炼焦炉工人尿中1-OHP和2-萘酚浓度高于学生，且与工作区域显著相关。学生尿中2-萘酚浓度随吸烟量增加而升高。炼焦炉工人空气中总PAH水平与尿中1-OHP浓度相关。CYP2E1基因c1/c2或c2/c2基因型的炼焦炉工人尿中1-OHP和2-萘酚浓度高于c1/c1基因型者。GSTM1缺失的工人尿中2-萘酚浓度高于GSTM1阳性的工人。在多元回归分析中CYP2E1是决定炼焦炉工人尿中1-OHP浓度的重要因素。CYP2E1和GSTM1是炼焦炉工人尿中2-萘酚浓度的重要决定因素，而GSTM1和吸烟是大学生尿中2-萘酚浓度的预测因素。对于炼焦炉工人，尿中1-OHP是职业性PAH暴露比2-萘酚更好的指标，而尿中2-萘酚对于非职业性PAH吸入暴露可能更敏感。在职业暴露人群中CYP2E1和GSTM1似乎在芘和萘代谢中起重要作用。在未职业暴露于PAHs的个体中，GSTM1和吸烟似乎影响尿中2-萘酚浓度。
