Pakhomova S, Kobayashi M, Buck J, Newcomer M E
Biochemistry Department, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-0146, USA.
Nat Struct Biol. 2001 May;8(5):447-51. doi: 10.1038/87617.
We report here the crystal structure of retinol dehydratase, an enzyme that catalyzes the synthesis of anhydroretinol. The enzyme is a member of the sulfotransferase superfamily and its crystal structure reveals the insertion of a helical lid into a canonical sulfotransferase fold. Site-directed mutations demonstrate that this inserted lid is necessary for anhydroretinol production but not for sulfonation; thus, insertion of a helical lid can convert a sulfotransferase into a dehydratase.
我们在此报告视黄醇脱水酶的晶体结构，该酶催化脱水视黄醇的合成。该酶是磺基转移酶超家族的成员，其晶体结构显示一个螺旋状盖子插入到典型的磺基转移酶折叠结构中。定点突变表明，这种插入的盖子对于脱水视黄醇的产生是必需的，但对于磺化不是必需的；因此，螺旋状盖子的插入可以将磺基转移酶转变为脱水酶。
