[离子色谱法同时分析酒类饮品（董酒）中的有机酸和无机阴离子]

[Simultaneous analysis of organic acids and inorganic anions in alcoholic drink (dongjiu) by ion chromatography].

作者信息

Ding M, Chen P, Luo G

机构信息

Department of Chemistry, Tsinghua University, Beijing, 100084.

出版信息

Se Pu. 1998 Jan;16(1):59-61.

PMID:11324482

Abstract

An ion chromatographic method for the simultaneous analysis of organic acids and inorganic anions in Dongjiu (alcoholic drink) was developed. An anion exchange column Shim-pack IC-A1(100 mm x 4.6 mm) was used. In non-suppressed anion exchange chromatography, potassium hydrogen phthalate (KHPh) is used as eluent most widely. However, there are several problems in this eluent for the analysis of Dongjiu (alcoholic drink) sample, such as the influence of a negative peak to the determination of acetic acid, ascorbic acid and lactic acid, the non-resolution of ascorbic acid, and lactic acid, and part overlapping of the peak of acetic acid with water peak. In order to solve these problems, a mixed eluent of 0.50 mmol/L KHPh and 0.25 mmol/L phthalic acid(H2Ph) was used in this work. The main organic acids and inorganic anions in Dongjiu were separated without interference peaks. The detection limits obtained by using the mixed eluent were 0.05-0.13 mg/L, several times lower than those obtained by using single KHPh eluent. This method, being without pretreatment, is simple to operate.

摘要

建立了一种同时分析董酒中有机酸和无机阴离子的离子色谱方法。使用了Shim-pack IC-A1阴离子交换柱（100 mm×4.6 mm）。在非抑制型阴离子交换色谱中，邻苯二甲酸氢钾（KHPh）是应用最广泛的洗脱剂。然而，对于董酒样品分析，该洗脱剂存在一些问题，比如负峰对乙酸、抗坏血酸和乳酸测定的影响，抗坏血酸和乳酸的分离度不够，以及乙酸峰与水峰部分重叠。为了解决这些问题，本研究采用了0.50 mmol/L KHPh和0.25 mmol/L邻苯二甲酸（H2Ph）的混合洗脱剂。董酒中的主要有机酸和无机阴离子得以分离，无干扰峰出现。使用混合洗脱剂获得的检测限为0.05 - 0.13 mg/L，比使用单一KHPh洗脱剂获得的检测限低几倍。该方法无需预处理，操作简便。