磷酸盐可刺激软骨祖细胞克隆ATDC5的分化和矿化。
Phosphate stimulates differentiation and mineralization of the chondroprogenitor clone ATDC5.
作者信息
Fujita T, Meguro T, Izumo N, Yasutomi C, Fukuyama R, Nakamuta H, Koida M
机构信息
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Japan.
出版信息
Jpn J Pharmacol. 2001 Mar;85(3):278-81. doi: 10.1254/jjp.85.278.
ATDC5 cells were employed to examine how inorganic phosphate (Pi) influences chondrocytic bone formation. 1) Pi (3 - 30 mM) plus ascorbic acid (50 microg/ml) dose-dependently accelerated proliferative differentiation and mineralization of ATDC5. 2) Northern blot analysis revealed that 10 mM Pi suppressed expression of type II collagen and PTH (parathyroid hormone) / PTH-related peptide (PTHrP) receptor, while it accelerated type X collagen expression. 3) Pi (3 - 30 mM) dose-dependently increased luciferase activity in the cells transfected with 3000 bp type X collagen promoter fused to the luciferase gene. The results suggest a regulatory role of Pi in endochondral osteogenesis.
采用ATDC5细胞来研究无机磷酸盐（Pi）如何影响软骨细胞骨形成。1）Pi（3 - 30 mM）加抗坏血酸（50微克/毫升）呈剂量依赖性地加速了ATDC5的增殖分化和矿化。2）Northern印迹分析显示，10 mM Pi抑制了II型胶原蛋白和甲状旁腺激素（PTH）/ PTH相关肽（PTHrP）受体的表达，同时加速了X型胶原蛋白的表达。3）Pi（3 - 30 mM）呈剂量依赖性地增加了转染了与荧光素酶基因融合的3000 bp X型胶原蛋白启动子的细胞中的荧光素酶活性。结果表明Pi在软骨内骨生成中具有调节作用。
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