苜蓿中华根瘤菌中存在CtrA细胞周期调节因子的同源物，且该同源物是必需的。
A homolog of the CtrA cell cycle regulator is present and essential in Sinorhizobium meliloti.
作者信息
Barnett M J, Hung D Y, Reisenauer A, Shapiro L, Long S R
机构信息
Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA.
出版信息
J Bacteriol. 2001 May;183(10):3204-10. doi: 10.1128/JB.183.10.3204-3210.2001.
Abstract
During development of the symbiotic soil bacterium Sinorhizobium meliloti into nitrogen-fixing bacteroids, DNA replication and cell division cease and the cells undergo profound metabolic and morphological changes. Regulatory genes controlling the early stages of this process have not been identified. As a first step in the search for regulators of these events, we report the isolation and characterization of a ctrA gene from S. meliloti. We show that the S. meliloti CtrA belongs to the CtrA-like family of response regulators found in several alpha-proteobacteria. In Caulobacter crescentus, CtrA is essential and is a global regulator of multiple cell cycle functions. ctrA is also an essential gene in S. meliloti, and it is expressed similarly to the autoregulated C. crescentus ctrA in that both genes have complex promoter regions which bind phosphorylated CtrA.
摘要
在共生土壤细菌苜蓿中华根瘤菌发育成固氮类菌体的过程中，DNA复制和细胞分裂停止，细胞经历深刻的代谢和形态变化。控制这一过程早期阶段的调控基因尚未被鉴定出来。作为寻找这些事件调控因子的第一步，我们报告了从苜蓿中华根瘤菌中分离和鉴定出一个ctrA基因。我们表明，苜蓿中华根瘤菌CtrA属于在几种α-变形杆菌中发现的CtrA样应答调节因子家族。在新月柄杆菌中，CtrA是必需的，并且是多种细胞周期功能的全局调节因子。ctrA在苜蓿中华根瘤菌中也是一个必需基因，它的表达与自我调节的新月柄杆菌ctrA相似，因为这两个基因都有复杂的启动子区域，可结合磷酸化的CtrA。
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