禽类退行性关节疾病期间关节软骨蛋白聚糖含量的变化
Changes in proteoglycan content of articular cartilage during avian degenerative joint disease.
作者信息
Anderson-MacKenzie J M, Robins S P, Thorp B H, Hulmes D J
机构信息
Department of Biochemistry, University of Edinburgh, Edinburgh, UK.
出版信息
Clin Exp Rheumatol. 2001 Mar-Apr;19(2):159-64.
OBJECTIVE
To determine the biochemical changes in articular cartilage composition associated with the development of avian degenerative joint disease (DJD) in ad libitum fed broiler fowl, in comparison to feed-restricted broilers and J-lin fowl (non-susceptible to DJD).
METHODS
Articular cartilage from the distal tibiotarsus (DTT) was characterised up to age 180 days. Proteoglycan content was determined by uronic acid and sulphated glycosaminoglycan analysis, cellularity by assay for DNA content, and collagen content and crosslinking by hydroxyproline and pyridinoline analysis, respectively.
RESULTS
Disease development was accompanied by increased hydration and proteoglycan content (particularly sulphated proteoglycans) and decreased cellularity, with no significant differences in either total collagen content or in mature collagen cross-linking.
CONCLUSION
The biochemical features of avian DJD are similar to those observed in other animal models. This bipedal model is exceptional however since cartilage alterations occur spontaneously and in a load-dependent manner.
目的
与限饲肉鸡和J系鸡（对退行性关节病不易感）相比，确定自由采食的肉鸡发生禽退行性关节病（DJD）时关节软骨成分的生化变化。
方法
对180日龄以下的胫跗关节远端（DTT）关节软骨进行特征分析。通过糖醛酸和硫酸化糖胺聚糖分析测定蛋白聚糖含量，通过DNA含量测定细胞密度，分别通过羟脯氨酸和吡啶啉分析测定胶原蛋白含量和交联情况。
结果
疾病发展伴随着水合作用增加和蛋白聚糖含量（特别是硫酸化蛋白聚糖）增加以及细胞密度降低，总胶原蛋白含量或成熟胶原蛋白交联均无显著差异。
结论
禽DJD的生化特征与其他动物模型中观察到的相似。然而，这种两足动物模型很特殊，因为软骨改变是自发发生的且与负荷有关。
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