Xie J, Chen J, Zhang F
Analysis & Testing Center, Sichuan Agriculture University, Ya'an, 625000.
Se Pu. 1998 May;16(3):258-60.
This paper describes a method for determining enrofloxacin (ERFX) and it's main metabolite ciprofloxacin(CPFX), in animal plasma by RP-IPLC simultaneously. Dichloromethane was utilized as an extracting agent of plasma. Chromatographic conditions were ODS column with methanol-25 mmol/L tetrabutylammonium hydroxide (20:80, V/V, pH 3.0) as mobile phase, pipemidic acid as the internal standard and UV detection at 272 nm. With the method established in this paper, the work is done simply, rapidly, sensitively and accurately. It was actually adapted to determine ERFX and it's metabolite CPFX concentrations in plasma and pharmacokinetic studies, and to determine ERFX and CPFX contents in calves plasma successfully for the first time.
本文描述了一种用反相离子对液相色谱法同时测定动物血浆中恩诺沙星（ERFX）及其主要代谢物环丙沙星（CPFX）的方法。采用二氯甲烷作为血浆萃取剂。色谱条件为：ODS柱，以甲醇-25 mmol/L氢氧化四丁铵（20:80，V/V，pH 3.0）为流动相，吡哌酸为内标，于272 nm处进行紫外检测。用本文建立的方法，操作简便、快速、灵敏、准确。该方法实际应用于血浆中ERFX及其代谢物CPFX浓度的测定和药代动力学研究，并首次成功测定了犊牛血浆中ERFX和CPFX的含量。
