Miyagawa S, Yamada M, Matsunami K, Koresawa Y, Ikawa M, Okabe M, Shirakura R
Division of Organ Transplantation, Osaka University Graduate School of Medicine, Yamadaoka, Suita, Osaka 565-0871, Japan.
J Biochem. 2001 May;129(5):795-801. doi: 10.1093/oxfordjournals.jbchem.a002922.
The human DAF (CD55) gene was chosen as a representative molecule in a xenotransplantation study. The gene was synthesized in order to adapt its codons to those which are more frequent in mammals, especially pigs, and the expression levels were then examined in Chinese hamster ovarian (CHO) cells, swine endothelial cell (SEC) and transgenic mice. A significant increase in protein production with no detectable mRNA elevation was observed in the transfectants of synthetic DAF (sDAF), compared with the wild-type DAF (wtDAF) and delta-SCR1 wild-type DAF (Delta1wtDAF). Consistent with the in vitro data, the expression of DAF in mice that carry sDAF was higher than Delta1wtDAF in many organs, especially the pancreas. The sDAF showed a high level of expression in SEC and transgenic mice, suggesting that it will be useful in the development of transgenic pigs with high levels of expression.
在一项异种移植研究中，人类衰变加速因子（CD55）基因被选为代表性分子。合成该基因是为了使其密码子适应在哺乳动物尤其是猪中更常见的密码子，然后在中国仓鼠卵巢（CHO）细胞、猪内皮细胞（SEC）和转基因小鼠中检测其表达水平。与野生型DAF（wtDAF）和δ-SCR1野生型DAF（Delta1wtDAF）相比，在合成DAF（sDAF）转染子中观察到蛋白质产量显著增加，而mRNA水平未检测到升高。与体外数据一致，携带sDAF的小鼠中DAF在许多器官尤其是胰腺中的表达高于Delta1wtDAF。sDAF在SEC和转基因小鼠中显示出高水平表达，表明它将有助于开发具有高表达水平的转基因猪。
