Lépine G, Ellen R P
University of Toronto, Faculty of Dentistry, Toronto, Ontario M5G 1G6, Canada.
DNA Seq. 2000;11(5):419-31. doi: 10.3109/10425170009033992.
The mglA and mglB genes (td-mglA and td-mglB) of the oral spirochete Treponema denticola were sequenced. These two T. denticola genes are highly homologous to the E. coli and Treponema pallidum mglA and mglB genes which are part of the three gene beta-methylgalactoside transport operon, mglBAC. Both Td-mglA and td-mglB are also homologous to the high affinity ABC-type transporters for ribose and arabinose, and surface presentation antigens (spa) locus, part of the type III secretion systems in enteropathogens. Td-mglB and td-mglA are co-transcribed as a single mRNA in T. denticola as well as in E. coli cells as determined by reverse transcription PCR (RT-PCR). Homology to td-mglB and its expressed protein was found in other oral spirochetes as determined by Southern and western blot analysis.
对口腔螺旋体齿垢密螺旋体的mglA和mglB基因（td - mglA和td - mglB）进行了测序。这两个齿垢密螺旋体基因与大肠杆菌和梅毒螺旋体的mglA和mglB基因高度同源，后者是三基因β - 甲基半乳糖苷转运操纵子mglBAC的一部分。Td - mglA和td - mglB也与核糖和阿拉伯糖的高亲和力ABC型转运体以及表面呈现抗原（spa）位点同源，该位点是肠道病原体中III型分泌系统的一部分。通过逆转录PCR（RT - PCR）确定，Td - mglB和td - mglA在齿垢密螺旋体以及大肠杆菌细胞中作为单一mRNA共转录。通过Southern和western印迹分析确定，在其他口腔螺旋体中发现了与td - mglB及其表达蛋白的同源性。
