Shah D, Aurora D, Lance R, Stuart G W
Department of Life Sciences, Indiana State University, Terre Haute, IN 47809, USA.
DNA Seq. 2000;11(5):457-61. doi: 10.3109/10425170009033997.
Previously undescribed POU genes were detected in several invertebrate phyla using redundant primers in a polymerase chain reaction (PCR) that targeted highly conserved sequences encoding known POU-domains. A class IV gene and a gene tentatively assigned to class VI were identified in sea anemones (Condylactis), two distinct class III genes were identified in snails (Biomphalaria), and a single class IV gene was identified in earthworms (Lumbricus). The identification of POU genes in cnidarians, mollusks, and annelids completes a survey of the major metozoan phyla. As POU genes exist in all of these organisms, they appear to be a fundamental characteristic of the metazoan lineage, and may have played a major role in the diversification of these organisms.
利用针对编码已知POU结构域的高度保守序列的冗余引物，在聚合酶链反应（PCR）中，在几个无脊椎动物门中检测到了以前未描述的POU基因。在海葵（Condylactis）中鉴定出一个IV类基因和一个暂时归为VI类的基因，在蜗牛（Biomphalaria）中鉴定出两个不同的III类基因，在蚯蚓（Lumbricus）中鉴定出一个IV类基因。刺胞动物门、软体动物门和环节动物门中POU基因的鉴定完成了对后生动物主要门类的调查。由于POU基因存在于所有这些生物中，它们似乎是后生动物谱系的一个基本特征，并且可能在这些生物的多样化过程中发挥了重要作用。
