通过在专门的结核微芯片上进行杂交和聚合酶链反应检测耐利福平结核分枝杆菌菌株
Detection of rifampicin-resistant Mycobacterium tuberculosis strains by hybridization and polymerase chain reaction on a specialized TB-microchip.
作者信息
Mikhailovich V M, Lapa S A, Gryadunov D A, Strizhkov B N, Sobolev A Y, Skotnikova O I, Irtuganova O A, Moroz A M, Litvinov V I, Shipina L K, Vladimirskii M A, Chernousova L N, Erokhin V V, Mirzabekov A D
机构信息
V. A. Engel'gardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
出版信息
Bull Exp Biol Med. 2001 Jan;131(1):94-8. doi: 10.1023/a:1017555318388.
Two alternative methods for identification of rifampicin-resistant strains of Mycobacterium tuberculosis on biological microchips are developed. The methods are based on detection of point mutations and other rearrangements in the rpoB gene region determining rifampicin resistance. Hybridization on TB-microchip detects 30 mutant variants of DNA in rifampicin-resistant strains (about 95% of all resistant forms). Allele-specific microchip PCR shortens the duration of analysis to 1.5 h. These methods can be used in clinical diagnostic laboratories for evaluating drug resistance/sensitivity of tuberculosis agent and for monitoring of the efficiency of antibiotic therapy.
开发了两种在生物微芯片上鉴定结核分枝杆菌耐利福平菌株的替代方法。这些方法基于检测决定利福平抗性的rpoB基因区域中的点突变和其他重排。在结核微芯片上进行杂交可检测耐利福平菌株中30种DNA突变变体（约占所有耐药形式的95%）。等位基因特异性微芯片PCR将分析时间缩短至1.5小时。这些方法可用于临床诊断实验室，以评估结核病菌的耐药性/敏感性，并监测抗生素治疗的效果。
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