Chen Z L, Iding K, Lütkemeyer D, Lehmann J
Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China.
Sheng Wu Gong Cheng Xue Bao. 2001 Jan;17(1):109-12.
A Super-Spinner was Modified by mounting a stainless steel filter(pore size 75 microns) to the impeller shaft to retain cells while fresh nutrient is perfused. Using Macroporous microcarrier Cytopore 1, continuously perfused cultivation of a recombinant CHO cell line, CHO2DS producing prothrombin was performed with the perfusion of a protein-free medium DF6S. The cell retention rate was more than 90% during the 24 days continuously perfused cultivation. The viable cell density of CHO2DS and prothrombin concentration reached 4.62 x 10(6)(cells.m/L) and 11.3(mg/L) respectively after 9 days culture.
通过在叶轮轴上安装一个不锈钢过滤器（孔径75微米）来改造一个超级旋转器，以便在灌注新鲜营养物质时留住细胞。使用大孔微载体Cytopore 1，在无蛋白培养基DF6S的灌注下，对生产凝血酶原的重组CHO细胞系CHO2DS进行连续灌注培养。在24天的连续灌注培养过程中，细胞保留率超过90%。培养9天后，CHO2DS的活细胞密度和凝血酶原浓度分别达到4.62×10(6)（细胞/毫升）和11.3（毫克/升）。
