Zhou J, Deng J X, Cheng X, Lu Y F, Yang X, Huang P T
Institute of Beijing Biotechnology, Beijing 100071, China.
Sheng Wu Gong Cheng Xue Bao. 2001 Jan;17(1):64-7.
In order to improve the expression of longer acting tissue plasminogen activator in the mammary epithelium of transgenic mice, the fragment of BLG-LAtPA hydrid gene was microinjected into mouse embryos with mice whey acid protein gene. Three mouse were tested as being Co-integration of BLG-LAtPA and WAP transgene by PCR and Southern blot. Milk obtained from lactating females contains biologically active tPA, and the concentration of tPA was calculated to be about 10 micrograms/mL.
为了提高长效组织纤溶酶原激活剂在转基因小鼠乳腺上皮中的表达，将BLG-LAtPA杂交基因片段与小鼠乳清酸蛋白基因一起显微注射到小鼠胚胎中。通过PCR和Southern印迹检测，三只小鼠被检测为BLG-LAtPA和WAP转基因的共整合。从泌乳雌性小鼠获得的乳汁中含有生物活性tPA，计算出tPA的浓度约为10微克/毫升。
