猪2号染色体（SSC2）的高分辨率比较RH图谱。

A high-resolution comparative RH map of porcine chromosome (SSC) 2.

作者信息

Rattink A P, Faivre M, Jungerius B J, Groenen M A, Harlizius B

机构信息

Department of Animal Sciences, Animal Breeding and Genetics Group, Wageningen Institute of Animal Sciences, Wageningen University, PO Box 338, 6700 AH, Wageningen, The Netherlands.

出版信息

Mamm Genome. 2001 May;12(5):366-70. doi: 10.1007/s003350020012.

DOI:10.1007/s003350020012

PMID:11331944

Abstract

A high-resolution comparative map was constructed for porcine Chromosome (SSC) 2, where a QTL for back fat thickness (BFT) is located. A radiation hybrid (RH) map containing 33 genes and 25 microsatellite markers was constructed for this chromosome with a 3000-rad porcine RH panel. In total, 16 genes from human Chromosome (HSA) 11p, HSA19p, and HSA5q were newly assigned to SSC2. One linkage group was observed at LOD 3.0, and five linkage groups at LOD 4.0. Comparison of the porcine RH map with homologous human gene orders identified four conserved segments between SSC2 and HSA11, HSA19, and HSA5. Concerning HSA11, a rearrangement of gene order is observed. The segment HSA11p15.4-q13 is inverted on SSC2 when compared with the distal tip of SSC2p, which is homologous to HSA11p15.5. The boundaries of the conserved segments between human and pig were defined more precisely. This high-resolution comparative map will be a valuable tool for further fine mapping of the QTL area.

摘要

构建了一张猪2号染色体（SSC2）的高分辨率比较图谱，背膘厚度（BFT）的一个数量性状基因座（QTL）位于该染色体上。利用一个3000拉德的猪辐射杂种（RH）细胞系构建了该染色体的一张包含33个基因和25个微卫星标记的RH图谱。总共将来自人类11号染色体（HSA）11p、HSA19p和HSA5q的16个基因新定位到了SSC2上。在LOD 3.0时观察到一个连锁群，在LOD 4.0时观察到五个连锁群。将猪的RH图谱与同源的人类基因顺序进行比较，确定了SSC2与HSA11、HSA19和HSA5之间的四个保守区段。关于HSA11，观察到了基因顺序的重排。与SSC2p的远端末端（与HSA11p15.5同源）相比，HSA11p15.4 - q13区段在SSC2上发生了倒位。更精确地界定了人和猪之间保守区段的边界。这张高分辨率比较图谱将成为进一步精细定位QTL区域的宝贵工具。