通过猪2号和16号染色体与人类染色体之间的高分辨率比较图谱确定的进化断点。

Evolutionary breakpoints through a high-resolution comparative map between porcine chromosomes 2 and 16 and human chromosomes.

作者信息

Lahbib-Mansais Yvette, Mompart Florence, Milan Denis, Leroux Sophie, Faraut Thomas, Delcros Chantal, Yerle Martine

机构信息

Institut National de la Recherche Agronomique, Laboratoire de Génétique Cellulaire, BP52627, 31326 Castanet-Tolosan, France.

出版信息

Genomics. 2006 Oct;88(4):504-12. doi: 10.1016/j.ygeno.2006.04.010. Epub 2006 Jun 9.

DOI:10.1016/j.ygeno.2006.04.010

PMID:16765019

Abstract

This study reports a high-resolution comparative map between human chromosomes and porcine chromosomes 2 (SSC2) and 16 (SSC16), pointing out new homologies and evolutionary breakpoints. SSC2 is of particular interest because of the presence of several important QTLs. Among 226 porcine ESTs selected according to their expected localization, 151 were RH mapped and ordered on SSC2. This study confirmed the extensive conservation between SSC2 and HSA11 and HSA19 and refined the homology with HSA5 (three blocks defined). Furthermore the SSC2q pericentromeric region was shown to be homologous to another human chromosome (HSA1). A complex organization of these syntenies was demonstrated on SSC2q. Our strategy led us to improve also the SSC16 RH map by adding 45 markers. Two-color fluorescence in situ hybridization of markers representative of each synteny confirmed block order. Finally, 29 breakpoints were identified in both species, and porcine BACs containing two breakpoints were isolated.

摘要

本研究报告了人类染色体与猪2号染色体（SSC2）和16号染色体（SSC16）之间的高分辨率比较图谱，指出了新的同源性和进化断点。由于存在几个重要的数量性状位点（QTL），SSC2特别引人关注。在根据预期定位选择的226个猪EST中，151个被进行了辐射杂种（RH）定位并排列在SSC2上。本研究证实了SSC2与人类11号染色体（HSA11）和19号染色体（HSA19）之间的广泛保守性，并细化了与人类5号染色体（HSA5）的同源性（定义了三个区段）。此外，还显示SSC2q着丝粒周围区域与另一人类染色体（HSA1）同源。在SSC2q上证明了这些同线性的复杂组织。我们的策略还使我们通过添加45个标记改进了SSC16的RH图谱。代表每个同线性的标记的双色荧光原位杂交证实了区段顺序。最后，在两个物种中鉴定出29个断点，并分离出含有两个断点的猪细菌人工染色体（BAC）。