大鼠视网膜祖细胞和一种视网膜色素上皮因子。

Rat retinal progenitor cells and a retinal pigment epithelial factor.

作者信息

Sheedlo H J, Brun-Zinkernagel A M, Oakford L X, Roque R S

机构信息

Department of Pathology and Anatomy, Division of Cell Biology and Genetics, University of North Texas Health Science Center, Fort Worth, TX 76107, USA.

出版信息

Brain Res Dev Brain Res. 2001 Apr 30;127(2):185-7. doi: 10.1016/s0165-3806(01)00118-3.

DOI:10.1016/s0165-3806(01)00118-3

PMID:11335005

Abstract

Retinal progenitor cells were isolated from explants of neonatal rat retinas and characterized by transmission electron microscopy and reverse transcriptase-polymerase chain reaction and by their response to an isolated retinal pigment epithelial cell cell factor. The isolated progenitor cells demonstrated nuclei with abundant euchromatin typical of progenitor cells and showed the presence of nestin and opsin message. A protein ( approximately 67 kDa) isolated from conditioned media of cultured rat RPE cells promoted the survival of isolated retinal progenitor cells.

摘要

视网膜祖细胞从新生大鼠视网膜外植体中分离出来，并通过透射电子显微镜、逆转录聚合酶链反应以及它们对一种分离的视网膜色素上皮细胞因子的反应进行表征。分离出的祖细胞显示出具有祖细胞典型的丰富常染色质的细胞核，并显示出巢蛋白和视蛋白信息的存在。从培养的大鼠视网膜色素上皮细胞的条件培养基中分离出的一种蛋白质（约67 kDa）促进了分离的视网膜祖细胞的存活。