从意大利南部羔羊和幼畜中分离出的瘟病毒的基因组特征分析。
Genomic characterization of pestiviruses isolated from lambs and kids in southern Italy.
作者信息
Pratelli A, Martella V, Cirone F, Buonavoglia D, Elia G, Tempesta M, Buonavoglia C
机构信息
Department of Health and Animal Well-being, University of Bari, St. p. per Casamassima Km 3, Valenzano, 70010, Bari, Italy.
出版信息
J Virol Methods. 2001 May;94(1-2):81-5. doi: 10.1016/s0166-0934(01)00277-4.
A nested polymerase chain reaction was used to identify 13 pestivirus strains isolated from small ruminants in several mixed (sheep and goats) flocks of Southern Italy, and for classification as bovine viral diarrhoea virus (BVDV) type 1, BVDV type 2, and Border disease virus (BDV) genotypes. Of the nine ovine isolates, two were characterized as BVDV type 1, and seven as BVDV type 2. The four pestiviruses isolated from kids belong to BVDV type 1. None of the pestivirus strains tested could be classified as 'true' BDV (genotype 3). Although BVDV type 2 has been described in Europe rarely, the characterization of BD/90-1M strain as BVDV type 2, isolated in Italy in 1990, demonstrates that this genotype has been circulating in Italy since the 1990s.
采用巢式聚合酶链反应对从意大利南部几个混合（绵羊和山羊）羊群的小反刍动物中分离出的13种瘟病毒株进行鉴定，并将其分类为牛病毒性腹泻病毒1型（BVDV-1）、牛病毒性腹泻病毒2型（BVDV-2）和边境病病毒（BDV）基因型。在9株绵羊分离株中，2株鉴定为BVDV-1型，7株为BVDV-2型。从羔羊中分离出的4种瘟病毒属于BVDV-1型。所检测的瘟病毒株均不能归类为“真正的”BDV（基因型3）。尽管BVDV-2在欧洲很少被描述，但1990年在意大利分离的BD/90-1M株被鉴定为BVDV-2型，这表明该基因型自20世纪90年代以来一直在意大利流行。
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