Choi Y E., Katsumi M, Sano H
Research and Education Center for Genetic Information, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, 630-0101, Nara, Japan
Plant Sci. 2001 May;160(6):1183-1190. doi: 10.1016/s0168-9452(01)00357-0.
The effect of auxin polar transport inhibitor on somatic embryo development and postembryonic growth in Siberian ginseng (Eleutherococcus senticosus) was examined. In the presence of 2,3,5-triiodobenzoic acid (TIBA), an auxin polar transport inhibitor, embryo formation from embryogenic cells was suppressed, while cell division was not affected. When globular embryos at different stages were transferred onto medium containing TIBA, development of axial and bilateral polarity was suppressed in a stagespecific manner. In abnormal embryos induced by TIBA, further development of shoot and root apical meristems and vascular differentiation was also suppressed. Thus, abnormal development of embryos induced by inhibition of auxin polar transport resulted in plantlets without shoots and roots.
研究了生长素极性运输抑制剂对西伯利亚人参（刺五加）体细胞胚胎发育和胚后生长的影响。在生长素极性运输抑制剂2,3,5-三碘苯甲酸（TIBA）存在的情况下，胚性细胞的胚胎形成受到抑制，而细胞分裂未受影响。当将不同阶段的球形胚转移到含有TIBA的培养基上时，轴向和双侧极性的发育以阶段特异性方式受到抑制。在TIBA诱导的异常胚中，茎尖和根尖分生组织的进一步发育以及维管分化也受到抑制。因此，生长素极性运输抑制诱导的胚异常发育导致形成没有茎和根的幼苗。
