碳氟化合物气溶胶推进剂V：与人类白蛋白的结合相互作用

Fluorocarbon aerosol propellants V: binding interaction with human albumin.

作者信息

Chiou W L, Hsiao J H

出版信息

J Pharm Sci. 1975 Jun;64(6):1052-4. doi: 10.1002/jps.2600640644.

Abstract

Binding of trichloromonofluoromethane, dichlorodifluoromethane, and dichlorotetrafluoroethane was studied in aqueous 5% human albumin solution, using the partition coefficient method in sealed serum bottles. The partition coefficient and the fraction of fluorocarbons bound were highly dependent on fluorocarbon concentrations. The average binding sites per molecule of albumin were 2.17, 0.30, and 0.42 and the binding association constants were 1.11 X10-3, 1.73 X10-3, and 5.06X10-3 M-minus 1, respectively. At the lowest concentration studied, 62.3, 25.5, and 65.6% were found bound to albumin, respectively. This appears to represent the first extensive study on any gas-albumin interaction.

摘要

采用密封血清瓶中的分配系数法，研究了三氯一氟甲烷、二氯二氟甲烷和二氯四氟乙烷在5%人血清白蛋白水溶液中的结合情况。氟碳化合物的分配系数和结合分数高度依赖于氟碳化合物的浓度。每分子白蛋白的平均结合位点分别为2.17、0.30和0.42，结合缔合常数分别为1.11×10⁻³、1.73×10⁻³和5.06×10⁻³M⁻¹。在所研究的最低浓度下，分别发现62.3%、25.5%和65.6%与白蛋白结合。这似乎是首次对任何气体-白蛋白相互作用进行的广泛研究。