文献检索，用中文搜 PubMed

Suppr 超能文献

核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Clinton M, Haines L, Belloir B, McBride D

Roslin Institute (Edinburgh), Roslin, Midlothian EH25 9PS, Scotland.

Br Poult Sci. 2001 Mar;42(1):134-8. doi: 10.1080/713655025.

Analysis of gene expression in the developing chick gonads requires the collection of male and female tissues from embryos between 3.5 d and 8.5 d of development. However, male and female chick embryos are indistinguishable by morphological examination before d 7.5 of development. 2. Sex identification of earlier embryos is only possible by molecular methods, which at present are laborious and time consuming. 3. We have devised a PCR-based sexing protocol which combines both sex specific and control reactions in a single tube assay. The assay is rapid and effective over a wide range of DNA concentrations and is tolerant of poor quality DNA. 4. Procedures are described for identifying the sex of individual embryos using either tissue samples or a small number of cells recovered from amniotic fluid.

分析发育中的鸡性腺中的基因表达需要从发育3.5天至8.5天的胚胎中采集雄性和雌性组织。然而，在发育7.5天之前，通过形态学检查无法区分雄性和雌性鸡胚胎。2. 早期胚胎的性别鉴定只能通过分子方法进行，而目前这些方法既费力又耗时。3. 我们设计了一种基于PCR的性别鉴定方案，该方案在单管检测中结合了性别特异性反应和对照反应。该检测在广泛的DNA浓度范围内快速有效，并且能够耐受低质量的DNA。4. 描述了使用组织样本或从羊水中回收的少量细胞来鉴定单个胚胎性别的程序。

Clinton M, Haines L, Belloir B, McBride D

Roslin Institute (Edinburgh), Roslin, Midlothian EH25 9PS, Scotland.

Br Poult Sci. 2001 Mar;42(1):134-8. doi: 10.1080/713655025.

Analysis of gene expression in the developing chick gonads requires the collection of male and female tissues from embryos between 3.5 d and 8.5 d of development. However, male and female chick embryos are indistinguishable by morphological examination before d 7.5 of development. 2. Sex identification of earlier embryos is only possible by molecular methods, which at present are laborious and time consuming. 3. We have devised a PCR-based sexing protocol which combines both sex specific and control reactions in a single tube assay. The assay is rapid and effective over a wide range of DNA concentrations and is tolerant of poor quality DNA. 4. Procedures are described for identifying the sex of individual embryos using either tissue samples or a small number of cells recovered from amniotic fluid.

分析发育中的鸡性腺中的基因表达需要从发育3.5天至8.5天的胚胎中采集雄性和雌性组织。然而，在发育7.5天之前，通过形态学检查无法区分雄性和雌性鸡胚胎。2. 早期胚胎的性别鉴定只能通过分子方法进行，而目前这些方法既费力又耗时。3. 我们设计了一种基于PCR的性别鉴定方案，该方案在单管检测中结合了性别特异性反应和对照反应。该检测在广泛的DNA浓度范围内快速有效，并且能够耐受低质量的DNA。4. 描述了使用组织样本或从羊水中回收的少量细胞来鉴定单个胚胎性别的程序。