Araake M, Hara T, Miyata A, Tani M, Ogawa H
Pharmaceutical Research Center, Meiji Seika Kaisha, LTD., 760 Morooka-cho, Kohoku-ku, Yokohama 222-8567, Japan.
Jpn J Antibiot. 2001 Feb;54(2):69-78.
We investigated the in vitro combination effects of arbekacin (ABK), vancomycin (VCM) or teicoplanin (TEIC) and cefepime (CFPM) on methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa isolated from various clinical specimens. Using checkerboard titration technique by agar dilution, combinations of ABK, VCM or TEIC and CFPM exhibited synergistic effects on 25 MRSA strains. The similar effects were also observed on ABK-resistant MRSA. Combination of ABK and CFPM exhibited a good effect on P. aeruginosa, but combinations of VCM or TEIC and CFPM exhibited no synergistic effect on P. aeruginosa. In vitro bactericidal activities of ABK, VCM or TEIC and CFPM against mixed cultures of MRSA with P. aeruginosa were examined at concentration of each drug that corresponds to the serum concentration at 3 hours after the usual therapeutic dosage. VCM or TEIC alone showed bacteriostatic effects against MRSA, and no enhancements were observed when combined with CFPM. ABK alone showed good bactericidal activity against MRSA and combination with CFPM enhanced the bactericidal activity. Against P. aeruginosa, ABK or CFPM alone showed the bactericidal activity, and strong bactericidal activity was induced by the combination of ABK and CFPM. VCM and TEIC showed no bactericidal activities against P. aeruginosa. When CFPM combined with VCM or TEIC, the bactericidal activity of CFPM was not enhanced against P. aeruginosa.
我们研究了阿贝卡星（ABK）、万古霉素（VCM）或替考拉宁（TEIC）与头孢吡肟（CFPM）对从各种临床标本中分离出的耐甲氧西林金黄色葡萄球菌（MRSA）和铜绿假单胞菌的体外联合效应。采用琼脂稀释棋盘滴定技术，ABK、VCM或TEIC与CFPM的联合用药对25株MRSA菌株显示出协同效应。在耐ABK的MRSA菌株上也观察到了类似的效应。ABK与CFPM联合用药对铜绿假单胞菌显示出良好的效果，但VCM或TEIC与CFPM联合用药对铜绿假单胞菌未显示出协同效应。在每种药物对应于常规治疗剂量后3小时血清浓度的浓度下，检测了ABK、VCM或TEIC与CFPM对MRSA与铜绿假单胞菌混合培养物的体外杀菌活性。单独使用VCM或TEIC对MRSA显示出抑菌作用，与CFPM联合使用时未观察到增强作用。单独使用ABK对MRSA显示出良好的杀菌活性，与CFPM联合使用可增强杀菌活性。对于铜绿假单胞菌，单独使用ABK或CFPM显示出杀菌活性，ABK与CFPM联合使用可诱导出较强的杀菌活性。VCM和TEIC对铜绿假单胞菌无杀菌活性。当CFPM与VCM或TEIC联合使用时，CFPM对铜绿假单胞菌的杀菌活性未增强。
