[在从墨西哥城一个动物园捕获的自由放养啮齿动物中寻找抗旋毛虫抗体]

[Search for antibodies against Trichinella spiralis in free roaming rodents caught in a zoological park from Mexico City].

作者信息

de-la-Rosa J L, Gómez-Priego A

机构信息

Departamento de Zoonosis, INDRE-SSA, Carpio 470, Santo Tomás, México D. F. 11340, México D. F. 11340, México.

出版信息

Bol Chil Parasitol. 2000 Jul-Dec;55(3-4):108-10.

PMID:11338971

Abstract

A serological survey to search for antibodies against T. spiralis was performed in free roaming rats (n = 64) and mice (n = 35) caught in a zoological park from Mexico City. Serum samples were analyzed by ELISA and immunoelectrotransfer blot assay (EIBT). None serum show positive absorbance values in ELISA nor recognized T. spiralis specific antigenic fractions in EIBT. However, two rat samples recognized three antigens of 31, 37 y 55 kDa, while one of them reacted with two additional antigens of 64 and 67 kDa. As it is known that the antigen epitope profiles varied among Trichinella species, it could be possible that in rats, there is 3% of antibody prevalence against Trichinella sp.; however, due that other organisms could induce the production of cross-reacting antibodies, such conclusion can not be supported at all. These results suggest that T. spiralis was not part of helminthological fauna in these rodents.

摘要

对从墨西哥城一个动物园捕获的自由放养大鼠（n = 64）和小鼠（n = 35）进行了一项血清学调查，以寻找抗旋毛虫的抗体。通过酶联免疫吸附测定（ELISA）和免疫电转移印迹分析（EIBT）对血清样本进行分析。在ELISA中，没有血清显示出阳性吸光度值，在EIBT中也没有血清识别出旋毛虫特异性抗原片段。然而，两份大鼠样本识别出了31、37和55 kDa的三种抗原，其中一份样本还与另外两种64和67 kDa的抗原发生了反应。由于已知旋毛虫属物种之间的抗原表位谱各不相同，有可能在大鼠中，抗旋毛虫属的抗体流行率为3%；然而，由于其他生物体可能诱导产生交叉反应抗体，因此该结论完全无法得到支持。这些结果表明，旋毛虫不是这些啮齿动物体内蠕虫动物区系的一部分。