Gulaya N M, Margitich V M, Govseeva N M, Klimashevsky V M, Gorpynchenko I I, Boyko M I
Department of Lipid Biochemistry, O.V. Palladin Institute of Biochemistry, National Academy of Sciences, Kiev, Ukraine.
Arch Androl. 2001 May-Jun;46(3):169-75. doi: 10.1080/01485010151096405.
The phospholipid and fatty acid composition of sperm was studied in 8 healthy and 16 infertile men. Infertile men randomly formed from the patients with normal semen parameters according to WHO criterion. Therefore, all semen parameters of infertile patients were similar to the same characteristics of the semen of healthy men, except the abnormal forms. The amount of abnormal forms in infertile men was significantly higher than in healthy men. Sperm from infertile men show a drastic loss of phosphatidyl ethanolamine. At the same time, the significant increase of phosphatidyl serine in the sperm and seminal plasma of sterile patients was found. Lysophosphatidyl serine in the sperm of the infertile men was detected. Fatty acid composition of the semen of infertile men was altered. The levels of stearic and n-3 polyunsaturated fatty acids (eicosopentaenoic and docosahexaenoic acids) was dramatically lowered, but the values of some n-6 polyunsaturated fatty acids (linolenic and docosatetraenoic) acids increased. There was significant positive correlation between docosahexaenoic acid and sperm motility (r = .82, p < .001) and negative correlation between linolenic acid and spermatozoa motility (r = -0.58. p < .05). Infertility of men with normal semen quality can originate from the disorder of sperm lipid metabolism. The drastic loss of phosphatidyl ethanolamine and n-3 polyunsaturated fatty acids with simultaneous enhancement of phosphatidyl serine and some n-6 polyunsaturated fatty acids in sperm could be an important cause of male infertility.
对8名健康男性和16名不育男性的精子磷脂和脂肪酸组成进行了研究。不育男性是根据世界卫生组织标准从精液参数正常的患者中随机选取的。因此，不育患者的所有精液参数与健康男性精液的相同特征相似，只是形态异常。不育男性的异常形态数量明显高于健康男性。不育男性的精子显示出磷脂酰乙醇胺的大量损失。同时，在不育患者的精子和精浆中发现磷脂酰丝氨酸显著增加。在不育男性的精子中检测到溶血磷脂酰丝氨酸。不育男性精液的脂肪酸组成发生了改变。硬脂酸和n-3多不饱和脂肪酸（二十碳五烯酸和二十二碳六烯酸）的水平显著降低，但一些n-6多不饱和脂肪酸（亚麻酸和二十二碳四烯酸）的值增加。二十二碳六烯酸与精子活力之间存在显著正相关（r = 0.82，p < 0.001），亚麻酸与精子活力之间存在负相关（r = -0.58，p < 0.05）。精液质量正常的男性不育可能源于精子脂质代谢紊乱。精子中磷脂酰乙醇胺和n-3多不饱和脂肪酸的大量损失，同时磷脂酰丝氨酸和一些n-6多不饱和脂肪酸增加，可能是男性不育的重要原因。
