Olami Y, Zajicek G, Cogan M, Gnessin H, Pe'er J
Department of Ophthalmology, Hadassah University Hospital and Hebrew University-Hadassah Faculty of Medicine, Jerusalem, Israel.
Ophthalmic Res. 2001 May-Jun;33(3):170-5. doi: 10.1159/000055665.
The object of this study is to determine the velocity and migration course of meibomian gland cells.
Twenty-seven adult rats weighing about 200 g were injected intraperitoneally with a single dose of 0.5 microCi [3H]-thymidine/g body weight. Three rats were sacrificed 1 h after injection and 3 rats each on days 3, 6, 9, 12, 15, 18, 21 and 28 after injection. The eyes and eyelids were removed in one piece, fixed in formalin, embedded in paraffin and cut into 5-micron-thick vertical sections along the pupillary-optic nerve axis. The sections were dipped in liquid autoradiographic emulsion, exposed for 3 weeks, and stained with HE. Meibomian glands were scanned in the lower eyelid of the left eye using an ocular micrometer grid. In each scan the basement membrane of each acinus served as a point of origin. Along the entire gland two parameters were noted for each nucleus with three grains or more: the distance from the basement membrane (Y) and grain count.
This study demonstrates for the first time the exact timing and direction of meibomian gland cell migration. We have shown that the cells migrate from the basement membrane to the center of the acinus at an average rate of 0.62 +/- 0.11 micron/day. Cell generation time was an average of 4.1 days.
The stem cells of meibomian glands lie at the circumference of each acinus. These cells migrate synchronously with a constant velocity towards the center of the acinus, and are secreted as sebaceous material.
本研究的目的是确定睑板腺细胞的速度和迁移过程。
27只体重约200 g的成年大鼠，每克体重腹腔注射单剂量0.5微居里的[3H] - 胸腺嘧啶核苷。注射后1小时处死3只大鼠，注射后第3、6、9、12、15、18、21和28天各处死3只大鼠。将眼球和眼睑完整摘除，固定于福尔马林中，石蜡包埋，并沿瞳孔 - 视神经轴切成5微米厚的垂直切片。切片浸入液体放射自显影乳剂中，曝光3周，并用苏木精 - 伊红染色。使用目镜测微网格在左眼的下眼睑中扫描睑板腺。在每次扫描中，每个腺泡的基底膜作为原点。沿着整个腺体，对每个有三个或更多银粒的细胞核记录两个参数：距基底膜的距离（Y）和银粒计数。
本研究首次证明了睑板腺细胞迁移的确切时间和方向。我们已经表明，细胞以平均0.62±0.11微米/天的速度从基底膜迁移到腺泡中心。细胞生成时间平均为4.1天。
睑板腺的干细胞位于每个腺泡的周边。这些细胞以恒定速度同步向腺泡中心迁移，并作为皮脂物质分泌。
