Feng X, Jiang H, Baik J C, Edgar C, Eide F F
Department of Neurology, University of Chicago, Chicago, Illinois, USA.
J Neurosci Res. 2001 May 15;64(4):355-63. doi: 10.1002/jnr.1086.
Neuroblastomas are heterogeneous tumors arising from sympathetic precursors in the neural crest. Growth factor stimulation of neuroblastomas promote diverse biological responses (mitogenesis, differentiation, cell death) depending on the particular tumor studied. Here we show that brief treatment with retinoic acid (RA) rendered the human neuroblastoma lines SY5Y, NGP, SMS-KCNR, and SK-N-SH dependent on brain-derived neurotrophic factor (BDNF) for survival. The BDNF- and trkB-expressing line SMS-KCN was dependent on an autocrine BDNF/trkB survival without exposure to RA. We conclude that the BDNF/trkB pathway plays an important role in neuroblastoma survival and speculate on a possible role in tumor pathogenesis.
神经母细胞瘤是起源于神经嵴交感神经前体细胞的异质性肿瘤。根据所研究的特定肿瘤，生长因子对神经母细胞瘤的刺激会引发多种生物学反应（有丝分裂、分化、细胞死亡）。在此，我们表明，用视黄酸（RA）进行短暂处理后，人神经母细胞瘤细胞系SY5Y、NGP、SMS-KCNR和SK-N-SH的存活依赖于脑源性神经营养因子（BDNF）。表达BDNF和trkB的细胞系SMS-KCN在未暴露于RA的情况下依赖自分泌BDNF/trkB存活。我们得出结论，BDNF/trkB信号通路在神经母细胞瘤存活中起重要作用，并推测其在肿瘤发病机制中可能发挥的作用。
