一种用于荧光法测定模拟过氧化物酶催化的H2O2的新型红色区域底物——四取代氨基铝酞菁。

A new red-region substrate, tetra-substituted amino aluminium phthalocyanine, for the fluorimetric determination of H2O2 catalyzed by mimetic peroxidases.

作者信息

Chen X L, Li D H, Yang H H, Zhu Q Z, Zheng H, Xu J G

机构信息

Key Laboratory of Analytical Sciences of MOE, Department of Chemistry, Xiamen University, Xiamen 361005, China.

出版信息

Analyst. 2001 Apr;126(4):523-7. doi: 10.1039/b009253n.

DOI:10.1039/b009253n

PMID:11340992

Abstract

A new red-region fluorogenic substrate, tetra-substituted amino aluminium pthalocyanine, was developed for the selective determination of H2O2 based on the catalytic effect of mimetic peroxidases, viz., hemin or iron tetrasulfonatophthalocyanine (FeTSPc). Under the optimum conditions, the linearity of the calibration graph for the determination of H2O2 with hemin (or FeTSPc) as the catalyst was in the range from 0.0 to 3.0 x 10(-7) mol L-1 (or from 0.0 to 2.0 x 10(-6) mol L-1). The detection limits were 3.7 x 10(-9) and 4.9 x 10(-9) mol L-1 H2O2, respectively. The relative standard deviation (n = 7) was within 1.5% in the middle of the linear range. The peroxidase activity of the mimetic enzymes hemin and FeTSPc, the effects of some experimental conditions and the influence of foreign substances were investigated. With this substrate, 0.0-7.5 x 10(-8) mol L-1 hemin and 0.0-2.0 x 10(-6) mol L-1 FeTSPc can be determined with an accuracy and precision of about 1.3%. The potential application of the reagent was tested by the determination of H2O2 in rainwater.

摘要

基于模拟过氧化物酶（即血红素或四磺酸基酞菁铁（FeTSPc））的催化作用，开发了一种新型红色区域荧光底物——四取代氨基铝酞菁，用于选择性测定过氧化氢。在最佳条件下，以血红素（或FeTSPc）为催化剂测定过氧化氢的校准曲线线性范围为0.0至3.0×10⁻⁷mol L⁻¹（或0.0至2.0×10⁻⁶mol L⁻¹）。检测限分别为3.7×10⁻⁹和4.9×10⁻⁹mol L⁻¹过氧化氢。在线性范围中间，相对标准偏差（n = 7）在1.5%以内。研究了模拟酶血红素和FeTSPc的过氧化物酶活性、一些实验条件的影响以及外来物质的影响。使用该底物，可以以约1.3%的准确度和精密度测定0.0至7.5×10⁻⁸mol L⁻¹的血红素和0.0至2.0×10⁻⁶mol L⁻¹的FeTSPc。通过测定雨水中的过氧化氢测试了该试剂的潜在应用。