Gunisha P, Madhavan H N, Jayanthi U, Therese K L
Microbiology Research Centre, Vision Research Foundations, Sankara Nathralaya, 18, College Road, Chennai.
Indian J Pathol Microbiol. 2000 Oct;43(4):395-402.
Nucleic acid amplification using IS6110 primers to detect Mycobacterium tuberculosis in clinical specimens has been extensively used as laboratory tool for the diagnosis for tuberculosis. Despite it's dramatic scientific value in practice, it is not as sensitive as expected for the detection of Mycobacterium tuberculosis. The results of the study suggest that PCR using 123 bp fragment of DNA belonging to IS6110 is specific (95.6%) but only has a sensitivity of 30% to detect M. tuberculosis in clinical specimens.
使用IS6110引物进行核酸扩增以检测临床标本中的结核分枝杆菌，已被广泛用作结核病诊断的实验室工具。尽管它在实践中具有巨大的科学价值，但在检测结核分枝杆菌方面并不像预期的那样敏感。研究结果表明，使用属于IS6110的123 bp DNA片段进行PCR具有特异性（95.6%），但在临床标本中检测结核分枝杆菌的灵敏度仅为30%。
