Roig Celma C, Palazón J, Cusidó R M, Piñol M T, Keil M
Departament de Productes Naturals, Biologia Vegetal i Edafologia, Facultat de Farmàcia, Universitat de Barcelona, Barcelona, Spain.
Planta Med. 2001 Apr;67(3):249-53. doi: 10.1055/s-2001-12006.
Hairy root cultures were obtained from hybrid clones of Duboisia myoporoides x D. leichhardtii following transformation by Agrobacterium rhizogenes strain A4. Shoots spontaneously regenerating from the hairy root cultures were rooted and transferred to soil. The plants displayed typical morphological alterations known as hairy root syndrome to varying degrees. PCR analysis confirmed that all transformed plants contained the rolA, rolB and rolC genes, irrespective of the degree of morphological alterations. A field test of the transformed regenerated plants revealed that those plants displaying the strongest hairy root syndrome symptoms had the highest content of the tropane alkaloid scopolamine. However, the overall scopolamine and hyoscyamine yield of all transformed plants was clearly reduced compared to untransformed control plants. These results demonstrate that the A. rhizogenes-transformed plants tested in this study do not provide a viable alternative to agricultural farming of hybrid clones of D. myoporoides x D. leichhardtii obtained by conventional breeding.
发根农杆菌菌株A4转化后，从洋金花（Duboisia myoporoides）与利氏洋金花（D. leichhardtii）的杂交克隆中获得了毛状根培养物。从毛状根培养物中自发再生的芽生根后转移到土壤中。这些植物不同程度地表现出典型的形态改变，即毛状根综合征。PCR分析证实，所有转化植株均含有rolA、rolB和rolC基因，与形态改变程度无关。对转化再生植株的田间试验表明，那些表现出最强毛状根综合征症状的植株，其托烷生物碱东莨菪碱含量最高。然而，与未转化的对照植株相比，所有转化植株的东莨菪碱和莨菪碱总产量明显降低。这些结果表明，本研究中测试的发根农杆菌转化植株并不能为通过传统育种获得的洋金花与利氏洋金花杂交克隆的农业种植提供可行的替代方案。
