枯草芽孢杆菌DNA中的链内自我互补序列。

Intrastrand self-complementary sequences in Bacillus subtilis DNA.

作者信息

Galloway D A, Rudner R

出版信息

J Gen Microbiol. 1979 Apr;111(2):353-61. doi: 10.1099/00221287-111-2-353.

Abstract

Intrastrand self-complementary sequences have been isolated from the DNA of Bacillus subtilis by hydroxyapatite (HA) chromatography following thermal renaturation of strands separated by chromatography on methylated albumin kieselguhr (MAK). The instrastrand structures derived from the MAK H strand (HA HII) were biologically active showing transforming activity for a wide variety of markers, as well as hybridization to both pulse-labelled and ribosomal RNA. Removal of regions of single-strand DNA with S1 nuclease did not significantly alter the biological activity of the self-annealed molecules. The overall efficiency of transformation and hybridization of the intrastrand self-annealing DNA was low suggesting that many sequences in the population are neither active in transformation to prototrophy nor transcribed into RNA.

摘要

通过甲基化白蛋白硅藻土（MAK）色谱法分离出的链在热复性后，利用羟基磷灰石（HA）色谱法从枯草芽孢杆菌的DNA中分离出链内自我互补序列。源自MAK H链（HA HII）的链内结构具有生物活性，对多种标记物显示出转化活性，并且能与脉冲标记的RNA和核糖体RNA杂交。用S1核酸酶去除单链DNA区域并没有显著改变自我退火分子的生物活性。链内自我退火DNA的转化和杂交总体效率较低，这表明群体中的许多序列既没有转化为原养型的活性，也没有转录成RNA。