转化脱氧核糖核酸在甲基化白蛋白上的色谱分析及凝胶过滤分析
Chromatography of transforming deoxyribonucleic acid on methylated albumin and by gel filtration.
作者信息
Barker G R, Hodges P
出版信息
Biochem J. 1974 Mar;137(3):543-6. doi: 10.1042/bj1370543.
Abstract
- Native DNA from two strains of Bacillus subtilis was chromatographed by stepwise elution from MAK (methylated albumin on kieselguhr). 2. Transforming activity was confined to two out of the three main fractions, activity being distributed between the two peaks differently for DNA from the different strains. 3. Fractionation of DNA from both strains on 2% agarose gel gave two components. Approx. 75% of the material was eluted within the void volume of the column. Approx. 25% of the material consisted of degradation products of lower molecular weight. 4. Chromatography on MAK of the material of high molecular weight eluted from agarose gel gave a number of peaks differing in molecular weight, indicating that degradation of the DNA takes place during chromatography on MAK. 5. The distribution of transforming activity among the fractions from MAK suggests that degradation occurs preferentially in certain regions of the DNA.
摘要
- 来自两株枯草芽孢杆菌的天然DNA通过从硅藻土甲基化白蛋白（MAK）上逐步洗脱进行色谱分析。2. 转化活性局限于三个主要组分中的两个，不同菌株的DNA在这两个峰之间的活性分布不同。3. 两株菌株的DNA在2%琼脂糖凝胶上分级分离得到两个组分。约75%的物质在柱的空体积内洗脱。约25%的物质由低分子量的降解产物组成。4. 从琼脂糖凝胶上洗脱的高分子量物质在MAK上进行色谱分析得到多个分子量不同的峰，表明DNA在MAK色谱分析过程中发生降解。5. MAK组分中转化活性的分布表明降解优先发生在DNA的某些区域。
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