日本人群中两个STR基因座D8S580和D22S442的等位基因结构与分布

Allelic structure and distribution of two STR loci, D8S580 and D22S442, in the Japanese population.

作者信息

Ota M, Katsuyama Y, Liu C, Takayanagi K, Asamura H, Fukushima H

机构信息

Department of Legal Medicine, Shinshu University School of Medicine, Asahi 3-1-1, 390-8621, Matsumoto, Japan.

出版信息

Forensic Sci Int. 2001 Jun 1;119(1):82-6. doi: 10.1016/s0379-0738(00)00411-4.

DOI:10.1016/s0379-0738(00)00411-4

PMID:11348797

Abstract

The allelic frequency and structural characteristics of two STR loci D8S580 and D22S442 were investigated using blood samples from 143 unrelated healthy Japanese individuals. Thirty-eight alleles in D8S580 locus and 13 alleles in D22S442 locus were identified. The discrimination power, heterozygosity, and the polymorphic information content of those loci displayed high values (0.98, 0.88, and 0.87 in D8S580 and 0.97, 0.86 and 0.85 in D22S442), and their frequency distributions met Hardy-Weinberg equilibrium expectations. The allelic pattern of D8S580 was complex and differentiated into three groups (group I: alleles 184-194bp; group II: alleles 203-223, 235, 239, 243, 252 and 255bp; group III: alleles 227-286bp). Most of their alleles contained five categories of repeat units (A: aaaag; B: aaag; C: aagg; D: caag; E: agaa). On the other hand, D22S442 contained only two types of repeat units (A: agga; B: aggg). The present study, hence, proves that both D8S580 and D22S442 are highly polymorphic and represent stable genetic markers applicable to forensic investigations.

摘要

利用143名无血缘关系的健康日本个体的血样，研究了两个STR基因座D8S580和D22S442的等位基因频率和结构特征。在D8S580基因座中鉴定出38个等位基因，在D22S442基因座中鉴定出13个等位基因。这些基因座的鉴别力、杂合性和多态信息含量显示出较高的值（D8S580分别为0.98、0.88和0.87，D22S442分别为0.97、0.86和0.85），并且它们的频率分布符合哈迪-温伯格平衡预期。D8S580的等位基因模式复杂，分为三组（第一组：184 - 194bp的等位基因；第二组：203 - 223、235、239、243、252和255bp的等位基因；第三组：227 - 286bp的等位基因）。它们的大多数等位基因包含五类重复单元（A：aaaag；B：aaag；C：aagg；D：caag；E：agaa）。另一方面，D22S442仅包含两种类型的重复单元（A：agga；B：aggg）。因此，本研究证明D8S580和D22S442均具有高度多态性，是适用于法医调查的稳定遗传标记。