培养介质对伯氏疏螺旋体基因调控模式的影响。
Influence of cultivation media on genetic regulatory patterns in Borrelia burgdorferi.
作者信息
Yang X, Popova T G, Goldberg M S, Norgard M V
机构信息
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75390, USA.
出版信息
Infect Immun. 2001 Jun;69(6):4159-63. doi: 10.1128/IAI.69.6.4159-4163.2001.
Abstract
Barbour-Stoenner-Kelly II (BSKII) medium and BSKH medium both are routinely used for the cultivation of Borrelia burgdorferi. However, heretofore there have been no studies to compare how these two media affect gene expression patterns in virulent B. burgdorferi. In the present study, we found that some B. burgdorferi strain 297 genes (e.g., ospA, mlp-7A, mlp-8, p22, and lp6.6) that typically are regulated by temperature or pH displayed their predicted pattern of expression when B. burgdorferi was cultivated in BSKH medium; this was not true when spirochetes were cultivated in conventional BSKII medium. The results suggest that BSKH medium is superior to BSKII medium for gene expression studies with B. burgdorferi.
摘要
巴伯-斯托纳-凯利II（BSKII）培养基和BSKH培养基都常用于培养伯氏疏螺旋体。然而，迄今为止，尚无研究比较这两种培养基如何影响毒力伯氏疏螺旋体的基因表达模式。在本研究中，我们发现，一些通常受温度或pH调节的伯氏疏螺旋体菌株297基因（如ospA、mlp-7A、mlp-8、p22和lp6.6），当伯氏疏螺旋体在BSKH培养基中培养时，呈现出其预测的表达模式；而当螺旋体在传统的BSKII培养基中培养时，情况并非如此。结果表明，在对伯氏疏螺旋体进行基因表达研究时，BSKH培养基优于BSKII培养基。
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