Rodríguez Islay, Lienhard Reto, Gern Lise, Veuve Marie Colette, Jouda Fatima, Siegrist Hans H, Fernández Carmen, Rodríguez José Enrique
National Laboratory of Spirochetes, Tropical Medicine Institute Pedro Kourí, Havana, Cuba.
Mem Inst Oswaldo Cruz. 2007 Dec;102(8):999-1002. doi: 10.1590/s0074-02762007000800017.
The aim of the present study was to assess the possible use of a modified medium, prepared in the laboratory using the constituents of Barbour-Stonner-Kelly (BSK) medium and medium 199 as base, for the culture of Borrelia strains, comparing the growth of individual strains in this medium and in the BSK-H medium, and the protein profile and antigenic characteristics of Borrelia proteins expressed in these media. A qualitative evaluation of growth of Borrelia species was made with acceptable results (morphology and motility), but during a quantitative evaluation using the three main genospecies of Borrelia, the better results were obtained with a B. burgdorferi sensu stricto strain. The modified medium did not enable the growth of a B. afzelii strain. The protein profile and antigenic characteristic of the expressed proteins in the modified medium were studied with satisfactory results. These results suggest the modified medium as an alternative for the cultivation of Borrelia strains, with some limitations, in poorly-resourced laboratories.
本研究的目的是评估一种改良培养基的潜在用途，该培养基是在实验室中以Barbour-Stonner-Kelly（BSK）培养基和199培养基的成分作为基础制备的，用于培养疏螺旋体菌株，比较各菌株在该培养基和BSK-H培养基中的生长情况，以及在这些培养基中表达的疏螺旋体蛋白的蛋白质谱和抗原特性。对疏螺旋体属细菌的生长进行了定性评估，结果可接受（形态和运动性），但在使用疏螺旋体的三个主要基因种进行定量评估时，严格意义上的伯氏疏螺旋体菌株获得了更好的结果。改良培养基不能使阿氏疏螺旋体菌株生长。对改良培养基中表达的蛋白质的蛋白质谱和抗原特性进行了研究，结果令人满意。这些结果表明，在资源匮乏的实验室中，改良培养基可作为培养疏螺旋体菌株的一种替代方法，但存在一些局限性。
