Roth J A, Frank D E, Weighner P, Weighner M
Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
J Dairy Sci. 2001 Apr;84(4):824-9. doi: 10.3168/jds.S0022-0302(01)74540-7.
The objective of this work was to evaluate a proprietary ultrafiltered bovine whey product for its in vitro influence on the function of neutrophils from normal and dexamethasone-treated cattle, and for its in vivo influence on neutrophil function in periparturient dairy cows. The ultrafiltered bovine whey was produced by hyperimmunizing cows to various bacterial pathogens by intramammary injection, collecting and pooling the colostrum and milk for the first 3 wk after parturition, then separating and processing the whey through a filter with a nominal molecular mass cut off of 10,000 Da. In vitro treatment of neutrophils from normal calves with ultrafiltered bovine whey significantly increased neutrophil random migration, cytochrome C reduction, iodination activity, antibody-dependent cell-mediated cytotoxicity, and antibody-independent cell-mediated cytotoxicity. Cytochrome C reduction was the only neutrophil function parameter significantly enhanced by the in vitro treatment of neutrophils from dexamethasone-treated cattle with ultrafiltered bovine whey. In vivo treatment of periparturient cows with ultrafiltered bovine whey did not alter the total or differential leukocyte counts in the animals but did significantly increase the total erythrocyte counts. In vivo treatment with ultrafiltered bovine whey also significantly increased neutrophil iodination activity in the periparturient cows. Neutrophil iodination activity (a measure of the myeloperoxidase/hydrogen peroxide/halide antibacterial system) is a very potent bactericidal mechanism of neutrophils and has previously been shown to be suppressed in periparturient cows.
本研究的目的是评估一种专利超滤牛初乳产品对正常牛和地塞米松处理牛中性粒细胞功能的体外影响，以及对围产期奶牛中性粒细胞功能的体内影响。超滤牛初乳是通过对奶牛进行乳房内注射，使其对各种细菌病原体进行超免疫，收集并汇集产后前3周的初乳和牛奶，然后通过截留分子量为10,000 Da的过滤器分离和处理乳清而制成的。用超滤牛初乳对正常犊牛的中性粒细胞进行体外处理，可显著增加中性粒细胞的随机迁移、细胞色素C还原、碘化活性、抗体依赖性细胞介导的细胞毒性和非抗体依赖性细胞介导的细胞毒性。细胞色素C还原是用地塞米松处理牛的中性粒细胞经超滤牛初乳体外处理后唯一显著增强的中性粒细胞功能参数。用超滤牛初乳对围产期奶牛进行体内处理，并未改变动物的白细胞总数或分类计数，但显著增加了红细胞总数。用超滤牛初乳进行体内处理还显著增加了围产期奶牛中性粒细胞的碘化活性。中性粒细胞碘化活性（髓过氧化物酶/过氧化氢/卤化物抗菌系统的一种测量指标）是中性粒细胞非常有效的杀菌机制，此前已证明在围产期奶牛中受到抑制。
