Madhavarao C N, Sauna Z E, Sitaramam V
Department of Biotechnology, University of Pune, India.
Biophys Chem. 2001 Apr 10;90(2):147-56. doi: 10.1016/s0301-4622(01)00136-3.
We monitored the fluorescence intensity and anisotropy of 1,6-diphenyl-1,3,5-hexatriene (DPH) incorporated in bovine serum albumin (BSA) and dimyristoylphosphatidylcholine (DMPC) vesicle membranes, which in turn were embedded in optically clear gelatin solutions, as a function of temperature. DPH in BSA gave unanticipated large changes in fluorescence intensity and anisotropy at the instant of gelatin gel melting. Both steady state anisotropy and fluorescence intensity reported the gel-sol transition point in gelatin unambiguously, which was independently confirmed as physical-pour point of the gel. In the case of DMPC vesicles, fluorescence intensity indicated the gelatin transition, while the anisotropy indicated DMPC phase transition. This fluorescence methodology uniquely offered a common probe for two distinct transitions in two distinct domains interconnected by the solvent, water.
