Park W Y, Hwang C I, Kang M J, Seo J Y, Chung J H, Kim Y S, Lee J H, Kim H, Kim K A, Yoo H J, Seo J S
Department of Biochemistry and Molecular Biology, Seoul National University, Seoul, Korea.
Biochem Biophys Res Commun. 2001 Apr 13;282(4):934-9. doi: 10.1006/bbrc.2001.4632.
In vitro cellular senescence of human diploid fibroblast has been a good model for aging research, which shows similar phenotypes to in vivo aging. Gene expression profiling would provide an insight to understand the mechanism of senescence. Using cDNA microarray containing 384 known genes, we compared the expression profiles of three different types of aging models: replicative senescence, fibroblasts from progeria or from elderly donor. Although all of them showed senescence phenotypes, distinct sets of genes were altered in each group. Pairwise plots or cluster analysis of activation fold of gene expression revealed closer relationships between fibroblasts from progeria or from old individual, but not between replicative senescence fibroblasts and either models. Differential expression pattern of several genes were confirmed by RT-PCR. We suggest that the replicative senescence model might behave differently to other types of aging models due to the distinct gene expression.
人二倍体成纤维细胞的体外细胞衰老一直是衰老研究的良好模型，其表现出与体内衰老相似的表型。基因表达谱分析将有助于深入了解衰老机制。我们使用包含384个已知基因的cDNA微阵列，比较了三种不同类型衰老模型的表达谱：复制性衰老、早老症患者的成纤维细胞或老年供体的成纤维细胞。尽管它们都表现出衰老表型，但每组中都有不同的基因集发生了改变。基因表达激活倍数的成对图或聚类分析显示，早老症患者或老年个体的成纤维细胞之间关系更密切，而复制性衰老的成纤维细胞与其他两种模型的成纤维细胞之间关系不密切。通过RT-PCR证实了几个基因的差异表达模式。我们认为，由于基因表达不同，复制性衰老模型的行为可能与其他类型的衰老模型不同。
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