Balzan S, Di Luca L, Taddei L, Salvador P, Montali U
CNR Istituto di Fisiologia Clinica, Pisa, Italy.
Cell Mol Biol (Noisy-le-grand). 2001 Mar;47(2):281-4.
3H-ouabain is useful to evaluate the tissue localization of Na,K-ATPase. In this work we determined the distribution of 3H-ouabain in rabbit tissue by digital radioautography. Using this method, we were able to obtain a comparison of various organs in a relatively short time (6.5 days), while with traditional radioautography, only the kidney was detectable after seven months of film exposure. The kidney has the highest intensity (concentration of 3H-ouabain), followed by the heart, liver, muscle, lung, spleen and finally brain, which was almost undetectable. In kidney the activity was higher in the cortex, while the other tissues displayed a more uniform intensity, suggesting that Na,K-ATPase was evenly distributed.
3H-哇巴因有助于评估钠钾-ATP酶的组织定位。在这项研究中，我们通过数字放射自显影法测定了3H-哇巴因在兔组织中的分布。使用这种方法，我们能够在相对较短的时间（6.5天）内对各种器官进行比较，而传统放射自显影法在胶片曝光7个月后只能检测到肾脏。肾脏的强度最高（3H-哇巴因浓度），其次是心脏、肝脏、肌肉、肺、脾脏，最后是大脑，几乎检测不到。在肾脏中，皮质的活性较高，而其他组织的强度更为均匀，这表明钠钾-ATP酶分布均匀。
