一种使用变性高效液相色谱法（DHPLC）通过异源双链分析进行性别鉴定的快速简便方法。
A rapid and simple method for sex identification by heteroduplex analysis, using denaturing high-performance liquid chromatography (DHPLC).
作者信息
Shinka T, Naroda T, Tamura T, Sasahara K, Nakahori Y
机构信息
Department of Public Health, School of Medicine, University of Tokushima, Japan.
出版信息
J Hum Genet. 2001;46(5):263-6. doi: 10.1007/s100380170076.
A novel method for sex identification, using a denaturing high-performance liquid chromatography (DHPLC) system, is described. Among many methods for identifying sex, the most popular and credible system has been the polymerase chain reaction (PCR) method, using nucleotide primer sets of the amelogenin gene, which is shared on both the X and Y chromosomes. With this conventional method, the judgment depends on detection of the size difference between the PCR products derived from the X and Y chromosomes. In this study, we adopted DHPLC to detect the difference by checking heteroduplex formation between the products, which enabled us to shorten the PCR products to 45bp and the separation time to within a period of 8min per sample. This new system may have wide applications in many different fields, such as forensic medicine, prenatal diagnosis, inbreeding of animals, and anthropology.
本文描述了一种利用变性高效液相色谱（DHPLC）系统进行性别鉴定的新方法。在众多性别鉴定方法中，最常用且可靠的系统是聚合酶链反应（PCR）法，该方法使用牙釉蛋白基因的核苷酸引物对，此基因在X和Y染色体上均有。采用这种传统方法时，判断依据是检测来自X和Y染色体的PCR产物之间的大小差异。在本研究中，我们采用DHPLC通过检查产物之间的异源双链形成来检测差异，这使我们能够将PCR产物缩短至45bp，每个样品的分离时间缩短至8分钟以内。这种新系统可能在许多不同领域有广泛应用，如法医学、产前诊断、动物近亲繁殖和人类学。
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