卡莫司汀对大鼠胶质瘤细胞中氨基芴 - DNA加合物形成的影响。

Effects of carmustine on aminofluorene-DNA adducts formation in rat glial tumor cells.

作者信息

Hung C F, Wong K T, Chung J G

机构信息

Department of Pediatrics, Jen-Ai Hospital, 483, Tong-Rong Road, Tali, Taichung, Taiwan.

出版信息

Acta Paediatr Taiwan. 2001 Mar-Apr;42(2):81-3.

PMID:11355069

Abstract

Arylamine-DNA adducts formation in tissues has been correlated with the carcinogenic effect of heterocyclic aromatic amines. Carmustine (BCNU) is a commercially available nitrosourea derivative and chloroethyl nitrosourea compound which is applied in anti-neoplastic therapy especially in central nervous system. The effects of carmustine on Aminofluorene-DNA (AF-DNA) formation in rat glioma cells were investigated by gamma-[32p]-dATP and high performance liquid chromatography (HPLC) using 2-aminofluorene (AF) as substrate. In the presence of 8 microM carmustine, the AF-DNA adduct formation was decreased 10.6% and 15.6% in 30 microM and 60 microM of AF (p < 0.05), respectively; In the presence of 80 microM carmustine the AF-DNA adduct formation was decreased 39.6% and 40% in 30 microM and 60 microM of AF (p < 0.02), respectively. These results show that BCNU could decrease AF-DNA formation in rat glial tumor cells.

摘要

组织中芳胺 - DNA加合物的形成与杂环芳香胺的致癌作用相关。卡莫司汀（BCNU）是一种市售的亚硝基脲衍生物和氯乙基亚硝基脲化合物，用于抗肿瘤治疗，尤其是在中枢神经系统。以2 - 氨基芴（AF）为底物，通过γ - [32P] - dATP和高效液相色谱（HPLC）研究了卡莫司汀对大鼠胶质瘤细胞中氨基芴 - DNA（AF - DNA）形成的影响。在存在8μM卡莫司汀的情况下，30μM和60μM的AF中AF - DNA加合物的形成分别降低了10.6%和15.6%（p < 0.05）；在存在80μM卡莫司汀的情况下，30μM和60μM的AF中AF - DNA加合物的形成分别降低了39.6%和40%（p < 0.02）。这些结果表明，BCNU可降低大鼠胶质瘤细胞中AF - DNA的形成。