Haven G T, Nguyen T T, Krzemien J R, Thuy L P
Res Commun Chem Pathol Pharmacol. 1975 Mar;10(3):473-90.
An inhibitor and stimulator of in vitro hepatic fatty acid synthesis are present in renal microsomes. In addition, a stimulator of fatty acid synthesis is present in renal lysosomes. Renal microsomal inhibition of hepatic fatty acid synthesis is not due to the depletion of cofactors in the system. This inhibitor appears to be located exclusively in the kidney medullary microsomes. It is destroyed by Pronase and heat treatment suggesting it may be a protein. Its effects on fatty acid synthesis may be attributed in part to ATPase activity as well as a direct effect on the hepatic fatty acid synthesizing system. A stimulator of hepatic fatty acid synthesis is present in the buffer insoluble fraction of an acetone powder preparation of renal microsomes. This stimulator is relatively heat labile and does not appear to be a phospholipid. The lysosomal stimulator of hepatic fatty acid synthesis is associated with the contents of renal lysosomes and not with the lysosomal membranes. It acts at the acetyl-CoA carboxylase step and its activity is not affected by fasting or aminonucleoside induced nephrosis.
肾微粒体中存在体外肝脂肪酸合成的抑制剂和刺激剂。此外，肾溶酶体中存在脂肪酸合成的刺激剂。肾微粒体对肝脂肪酸合成的抑制并非由于系统中辅因子的耗尽。这种抑制剂似乎仅位于肾髓质微粒体中。它可被链霉蛋白酶和热处理破坏，提示其可能是一种蛋白质。其对脂肪酸合成的作用可能部分归因于ATP酶活性以及对肝脂肪酸合成系统的直接作用。肝脂肪酸合成的刺激剂存在于肾微粒体丙酮粉制剂的缓冲液不溶性部分中。这种刺激剂相对不耐热，且似乎不是磷脂。肝脂肪酸合成的溶酶体刺激剂与肾溶酶体的内容物相关，而与溶酶体膜无关。它作用于乙酰辅酶A羧化酶步骤，其活性不受禁食或氨基核苷诱导的肾病影响。
