Chen T C, Chou T B, Ng K F, Hsieh L L, Chou Y H
Department of Pathology, Chang Gung Memorial Hospital, 5 Fu Shin Street, Kwei San, Tao Yuan, Taiwan.
Virchows Arch. 2001 Apr;438(4):408-11. doi: 10.1007/s004280000348.
We describe a hepatocellular carcinoma partially surrounded by focal nodular hyperplasia in a 65-year-old female patient. In order to clarify the relationship of the hepatocellular carcinoma and the adjacent focal nodular hyperplasia, clonal analysis was conducted. The clonal analysis was based on the methylation pattern of the polymorphic X-chromosome-linked androgen receptor gene (HUMARA). The allelic bands from the amplification of the focal nodular hyperplasia and of the hepatocellular carcinoma showed a significant reduction in the intensity of one of the two alleles as compared with two alleles of equal intensity in the buff coat after HhaI digestion, which indicated that these two parts were monoclonal. However, the inactivated allele in the focal nodular hyperplasia and that in the hepatocellular carcinoma were not identical. Therefore, the focal nodular hyperplasia and hepatocellular carcinoma probably derived from the clonal expansion of two different clones.
我们描述了一名65岁女性患者，其肝细胞癌部分被局灶性结节性增生所包围。为了阐明肝细胞癌与相邻局灶性结节性增生的关系，进行了克隆分析。克隆分析基于多态性X染色体连锁雄激素受体基因（HUMARA）的甲基化模式。与经HhaI消化后的血沉棕黄层中强度相等的两个等位基因相比，局灶性结节性增生和肝细胞癌扩增后的等位基因条带显示两个等位基因之一的强度显著降低，这表明这两个部分是单克隆的。然而，局灶性结节性增生中的失活等位基因与肝细胞癌中的失活等位基因并不相同。因此，局灶性结节性增生和肝细胞癌可能源自两个不同克隆的克隆性扩增。
