Slingsby R W, Bordunov A, Grimes M
Dionex Corporation, Sunnyvale, CA 94086, USA.
J Chromatogr A. 2001 Apr 13;913(1-2):159-63. doi: 10.1016/s0021-9673(00)01203-6.
An in-line stripper column packed with iminodiacetate chelation resin is placed between the pump and injection valve and shown to remove metallic impurities from an HPLC system. We used a test procedure based on assessing the relative peak asymmetries of 2,2'-bipyridyl, a chelating analyte and 4,4'-bipyridyl, a non-chelating analyte. Results from use of polyether ether ketone (PEEK) and stainless steel pumps are evaluated. Analytical columns with titanium/PEEK and stainless steel frits are used to assess the role of frits in metallic contamination. We demonstrated that although metal-free pumping systems contribute significantly lower metallic impurities than stainless steel systems, metal is nevertheless present in the mobile phase and the chelating stripper columns were found useful in protecting the analytical columns from contamination. The stainless steel frits were not found to be significant contributors to the contamination.
在泵和进样阀之间放置一个填充亚氨基二乙酸螯合树脂的在线剥离柱，结果表明该柱可去除高效液相色谱（HPLC）系统中的金属杂质。我们采用了一种测试程序，该程序基于评估螯合分析物2,2'-联吡啶和非螯合分析物4,4'-联吡啶的相对峰不对称性。对使用聚醚醚酮（PEEK）泵和不锈钢泵的结果进行了评估。使用带有钛/PEEK和不锈钢烧结板的分析柱来评估烧结板在金属污染中的作用。我们证明，尽管无金属的泵系统比不锈钢系统产生的金属杂质要少得多，但流动相中仍然存在金属，并且发现螯合剥离柱有助于保护分析柱免受污染。未发现不锈钢烧结板是造成污染的主要因素。
